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pbi gfp bub1 wt backbone  (Addgene inc)


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    Structured Review

    Addgene inc pbi gfp bub1 wt backbone
    Pbi Gfp Bub1 Wt Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi+gfp+bub1+wt+backbone/pBI-GFP-Bub1-wt+(Plasmid+%2316624)/pm40180891-444-14-16
    Average 93 stars, based on 2 article reviews
    pbi gfp bub1 wt backbone - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma.
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Sequencing:

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma.
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Plasmid Preparation:

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma.
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Cloning:

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma.
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Over Expression:

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..

    Article Title: Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma.
    Article Snippet: .. To achieve stable ectopic expression, the human BUB1 coding sequence was extracted from the pBI-GFP-Bub1-wt backbone (Addgene plasmid #16624) using NotI (R3189S, NEB) and BamHI (R3136S, NEB) restriction enzymes, and subcloned into the multiple cloning site (MCS) of the lentiviral overexpression vector, pHIV-Zsgreen (Addgene plasmid #18121). ..



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    93
    Addgene inc pbi gfp bub1 wt backbone
    Pbi Gfp Bub1 Wt Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi+gfp+bub1+wt+backbone/pBI-GFP-Bub1-wt+(Plasmid+%2316624)/pm40180891-444-14-16
    Average 93 stars, based on 1 article reviews
    pbi gfp bub1 wt backbone - by Bioz Stars, 2026-09
    93/100 stars
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